Processes and intermediate for the large-scale preparation of 2,4,6-trifluoro-n-[6-(1-methyl-piperidine-4-carbonyl)-pyridin-2-yl]-benzamide hemisuccinate, and preparation of 2,4,6-trifluoro-n-[6-(1-methyl-piperidine-4-carbonyl)-pyridin-2-yl]-benzamide acetate

ABSTRACT

The embodiments of present invention provide processes and an intermediate for the large-scale preparation of 2,4,6-tri-fluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide hemisuccinate, and formulations and product forms made by these processes. The embodiments of the present invention further provide for the preparation of lasmiditan acetate, 2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide acetate salt, and/or pharmaceutical compositions thereof, and/or uses of lasmiditan acetate and formulations thereof in subcutaneous drug delivery.

The embodiments of the present invention relate to the fields ofpharmaceutical chemistry and synthetic organic chemistry, and provideprocesses and an intermediate for the large-scale synthesis of2,4,6-trifluoro-N-[6-(1-methyl-piperidine-4-carbonyl)-pyridin-2-yl]-benzamidehemi-succinate salt, a 5-HT1F receptor agonist, and formulations andproduct forms made by these processes, and to preparation of2,4,6-trifluoro-N-[6-(1-methyl-piperidine-4-carbonyl)-pyridin-2-yl]-benzamideacetate and uses thereof for parenteral formulations and treatment ofmigraine.

Lasmiditan is a selective and highly potent 5-HT_(1F) receptor agonistwhich is now approved in the United States, as 50 mg or 100 mg tablets,for acute on-demand treatment of migraine (See e.g. Rubio-Beltrán etal., Pharmacol Ther 2018; 186:88-97, and Lasmiditan for the Treatment ofMigraine, Capi, M. et al., Expert Opinion Investigational Drugs, (2017),Vol. 26, NO. 2, 227-234). Lasmiditan (COL 144, LY 573144, CAS RegistryNo. 439239-90-4) can be described chemically as2,4,6-trifluoro-N-[6-(1-methyl-piperidin-4-ylcarbonyl)-pyridin-2-yl]-benzamide.U.S. Pat. No. 7,423,050 and U.S. Publication No. 20080300407 describethe hemisuccinate salt of2,4,6-trifluoro-N-[6-(1-methyl-piperidine-4-carbonyl)-pyridin-2-yl]-benzamidehaving the structural formula:

Methods of preparing lasmiditan and salts and certain polymorphic forms,formulations, and dosage forms thereof, are known to the skilledartisan, and are described for example in WO 2003/084949, WO2011/123654, and WO 2018/106657.

As used herein, useful forms of lasmiditan include pharmaceuticallyacceptable salts thereof, including but not limited to2,4,6-trifluoro-N-[6-(1-methyl-piperidin-4-ylcarbonyl)-pyridin-2-yl]-benzamidemono-hydrochloride salt, and2,4,6-trifluoro-N-[6-(1-methyl-piperidine-4-carbonyl)-pyridin-2-yl]-benzamidehemi-succinate salt. A synthetic route for the preparation of thehemi-succinate salt of2,4,6-trifluoro-N-[6-(1-methyl-piperidine-4-carbonyl)-pyridin-2-yl]-benzamidehas been disclosed previously as shown below in Scheme A. The overallyield of lasmiditan starting with commercially available piperidine4-carboxylic acid via the route described in Scheme A below is about10-46% over all 9 steps. Improvements in the synthesis of lasmiditancould provide substantial and varied benefits, particularly forproduction at large-scale.

Synthetic chemistry process routes may be redesigned or revised aimingto achieve various advantages, including for example: improved yields,obtaining crystalline products, decreasing impurity profiles, utilizingcommercially available intermediates, minimizing the number of syntheticsteps needed, reducing the inputs required and/or the by-productsproduced, or any useful combination of such improvements, to achieveimportant real-world outcomes including decreased costs, providing lessresource intensive processes, and facilitating efficient production.Improved methods of making lasmiditan are needed which may achieve oneor more of these aims, particularly for large-scale synthesis.

Further, migraine is one of the most common presenting symptoms inemergency rooms. Current methods for headache relief in the emergencyroom setting, when using lasmiditan for patients who have difficultyadministering a tablet due to nausea and/or vomiting, may need to relyon the preparation of a diluted formulation of about 1 mg/ml lasmiditandelivered intravenously over an extended period of time, for examplefrom about 20-60 minutes. Lasmiditan has been delivered intravenously inclinical studies in doses from about 1-60 mg delivered in 60 mlinfusions over 20 minutes (See US Patent Application Publication No.2010/0256187). The safe and effective treatment of migraine withlasmiditan for patients unable to administer tablets would be enabled bythe availability of a high concentration parenteral dosage form. Thepresent disclosure also addresses this need.

SUMMARY

The embodiments of the present invention provide processes for thepreparation of lasmiditan hemisuccinate,2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehemisuccinate salt, and/or compositions thereof, and/or particularlyuseful intermediates for use in these processes. The embodiments of thepresent invention further provide for the preparation of lasmiditanacetate,2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamideacetate salt, and/or compositions thereof, and/or uses of lasmiditanacetate, and formulations thereof, in subcutaneous drug delivery.

In one embodiment, referred to as Route I, the present inventionprovides a process for preparing a compound of the formula:

comprising the steps of:

-   -   i.) Treatment of piperidine-4-carboxylic acid under reductive        amination conditions comprising formaldehyde and formic acid in        water with subsequent treatment with aqueous HCl followed by        water distillation and acetonitrile addition, with repeated        dilution/distillation until the water content is not more than        0.2% by Karl-Fischer analysis, to obtain solid        1-methylpiperidine-4-carboxylic acid hydrochloride;    -   ii.) Treatment of 1-methylpiperidine-4-carboxylic acid        hydrochloride with a chlorinating agent such as thionyl chloride        in chlorobenzene obtain 1-methylpiperidine-4-carboxylic acid        chloride;    -   iii.) Treatment of 1-methylpiperidine-4-carboxylic acid chloride        with N,N-diethylamine in chlorobenzene containing triethylamine        with subsequent base wash and subsequent treatment with aqueous        HCl in isopropanol to obtain solid        N,N-diethyl-1-methyl-piperidine-4-carboxamide hydrate        hydrochloride;    -   iv.) Treatment of N,N-diethyl-1-methyl-piperidine-4-carboxamide        hydrate hydrochloride with a mineral base such as aqueous NaOH        in a non-polar solvent such as methyl-tert-butyl ether with        subsequent water wash, phase separation, and distillation of the        organic solvent until the water content is not more than 0.1        weight % by Karl Fischer analysis to obtain        N,N-diethyl-1-methyl-piperidine-4-carboxamide;    -   v.) Subsequent treatment of        N,N-diethyl-1-methyl-piperidine-4-carboxamide with        (6-bromo-2-pyridyl)lithium in a non-polar organic solvent such        as methyl-tert-butyl ether with subsequent extraction of the        resulting mixture with water and a suitable organic solvent such        as n-butanol, phase separation, and repeated distillation of the        organic solvent until the water content is not more than 0.2        weight % by Karl-Fischer analysis, to obtain        (6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone;    -   vi.) Treatment of        (6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone with aqueous        HBr and subsequent extraction with n-butanol followed by        repeated distillation of the organic solvent until the water        content is not more than 0.3% by Karl-Fischer analysis, to        obtain solid (6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone        hydrobromide;    -   vii.) Treatment of        (6-bromo-2-pyridyl-1-methyl-4-piperidyl)methanone hydrobromide        with a solution of NH₃ in ethylene glycol in the presence of        Cu₂O catalyst at about 80° C. for about 2 hr, with subsequent        washes with water, saturated aqueous NaCl, and 20% aqueous NaOH        and subsequent extraction with a non-polar aprotic solvent such        as methyl-tert-butyl ether, phase separation, and treatment of        the organic phase with 5 weight % carbon;    -   viii.) Filtration of the above mixture, dilution with a suitable        polar alcoholic solvent such as isopropanol, and repeated        distillation of the organic solvent until the water content is        not more than 0.2% by Karl-Fischer analysis, with subsequent        treatment of the resulting residue with isopropanol, water, and        20 weight % HCl, wherein the water concentration of the        resulting slurry is at least 2%, filtration of the resulting        slurry, and drying under vacuum at 40° C. for 16-24 hr to obtain        solid (6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone        dihydrate dihydrochloride;    -   ix.) Treatment of        (6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone dihydrate        dihydrochloride in chlorobenzene with 6 weight/weight % NaOH in        water at about 54° C. for about 30 min, with subsequent phase        separation and vacuum distillation of the aqueous solution to        obtain (6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone;    -   x.) Subsequent treatment of        (6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone with        2,4,6-trifluorobenzoic acid chloride in chlorobenzene at about        100° C. for about 4 hr, with subsequent cooling, charging with        acetonitrile and heating the resulting slurry to 80° C. for        about 1 hr, and subsequent collection of the resulting solid by        filtration, to obtain solid        2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide        hydrochloride;    -   xi.) Treatment of        2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide        hydrochloride with saturated aqueous Na₂CO₃ in methyl-tert-butyl        ether;    -   xii.) Treatment of the mixture of step xi above with SiO₂ with        subsequent filtration, treatment with carbon, filtration, and        evaporation, dilution with ethanol, and distillation until the        water content is not more than 1% by Karl-Fischer analysis, to        obtain        2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide;    -   xiii.) Treatment of        2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide        in ethanol with a solution of 0.5 equivalents succinic acid in        ethanol at about 55° C. for not less than 3 hr at RT, and        subsequent collection of the solid by filtration, to obtain        solid        2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide        hemisuccinate.

In the process of Route I above, preferably the reactions are performedusing batch processing methodology. In an embodiment the batches byRoute I are produced at process scale. In an embodiment the batches byRoute I are produced in at least 1 kilogram. In an embodiment thebatches by Route I are produced in at least 10 kilograms. In anembodiment the batches by Route I are produced in at least 100kilograms.

In the process of Route I above, the use of chlorobenzene avoidsdegradation which occurs under alternative methods, such as THF, whichreacts with the acid chloride under scale (e.g., 100 kg) resulting inessentially no yield of the acid chloride.

In another embodiment, referred to as Route II, the present inventionprovides a process for preparing a compound of the formula:

comprising the steps of:

-   -   i.) Treatment of piperidine-4-carboxylic acid under reductive        amination conditions comprising formaldehyde and formic acid in        water with subsequent treatment with aqueous HCl followed by        water distillation and acetonitrile addition, with repeated        dilution/distillation until the water content is not more than        0.2% by Karl-Fischer analysis, to obtain solid        1-methylpiperidine-4-carboxylic acid hydrochloride;    -   ii.) Treatment of 1-methylpiperidine-4-carboxylic acid        hydrochloride with a chlorinating agent such as thionyl chloride        in chlorobenzene to obtain 1-methylpiperidine-4-carboxylic acid        chloride;    -   iii.) Treatment of 1-methylpiperidine-4-carboxylic acid chloride        with N,N-diethylamine in chlorobenzene containing triethylamine        with subsequent base wash and subsequent treatment with aqueous        HCl in isopropanol to obtain solid        N,N-diethyl-1-methyl-piperidine-4-carboxamide hydrate        hydrochloride;    -   iv.) Treatment of N,N-diethyl-1-methyl-piperidine-4-carboxamide        hydrate hydrochloride with a mineral base such as aqueous NaOH        in a non-polar solvent such as methyl-tert-butyl ether with        subsequent water wash, phase separation, and distillation of the        organic solvent until the water content is not more than 0.1        weight % by Karl Fischer analysis to obtain        N,N-diethyl-1-methyl-piperidine-4-carboxamide;    -   v.) Subsequent treatment of        N,N-diethyl-1-methyl-piperidine-4-carboxamide with        (6-bromo-2-pyridyl)lithium in a non-polar organic solvent such        as methyl-tert-butyl ether with subsequent extraction of the        resulting mixture with water and a suitable organic solvent such        as n-butanol, phase separation, and repeated distillation of the        organic solvent until the water content is not more than 0.2        weight % by Karl-Fischer analysis, to obtain        (6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone;    -   vi.) Treatment of        (6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone with aqueous        HBr and subsequent extraction with n-butanol followed by        repeated distillation of the organic solvent until the water        content is not more than 0.3% by Karl-Fischer analysis, to        obtain solid (6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone        hydrobromide;    -   vii.) Treatment of        (6-bromo-2-pyridyl-1-methyl-4-piperidyl)methanone hydrobromide        in a biphasic mixture of water and toluene with solid KOH for        about 3 hr with subsequent separation of the organic layer and        evaporation of the solvent to obtain of        (6-bromo-2-pyridyl-1-methyl-4-piperidyl)methanone;    -   viii.) Treatment of        (6-bromo-2-pyridyl-1-methyl-4-piperidyl)methanone with        2,4,6-trifluorobenzamide in toluene containing K₂CO₃, water,        Pd(OAc)₂, and Xantphos at about 70° C. for about 12 hr, until        the (6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone content        is not more than 0.1% by HPLC, with subsequent dilution of the        reaction mixture with water and EtOAc, subsequent treatment with        thiourea-modified silica gel at 60° C. for about 8 hr, with        subsequent filtration to obtain a solution of        2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide;    -   ix.) Treatment of a solution of        2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide        in EtOAc with a solution of about 0.5 equivalents of succinic        acid dissolved in EtOH at 55° C. for about 3 hr, with subsequent        cooling to RT over about 10 hr, and collection of the resulting        solids by filtration, to obtain solid        2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide        hemisuccinate.

In the process of Route II above, preferably the reactions are performedusing batch processing methodology. In an embodiment the batches byRoute II are produced at process scale. In an embodiment the batches byRoute II are produced in at least 1 kilogram. In an embodiment thebatches by Route II are produced in at least 10 kilograms. In anembodiment the batches by Route II are produced in at least 100kilograms.

In the process of Route II above, the use of chlorobenzene avoidsdegradation which occurs under alternative methods, such as THF, whichreacts with the acid chloride under scale (e.g. 100 kg) resulting inessentially no yield of the acid chloride.

In another embodiment the present invention provides:

which can be named as(6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone dihydratedihydrochloride. Preferably this compound is crystalline.(6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone dihydratedihydrochloride is particularly useful in the preparation of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehemisuccinate, and processes which employ(6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone dihydratedihydrochloride may provide advantageous process characteristics,including but not limited to the purity of intermediate and/or finalmaterials. (6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone dihydratedihydrochloride is believed to be a new stable hydrated form of6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone. The process toisolate (6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone dihydratedihydrochloride described herein provides improved impurity rejectionand an improved controlled crystallization process. Form and chemicalstability studies showed(6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone dihydratedihydrochloride is generally stable, and drying studies show thatover-drying to remove water of hydration is difficult, even underforcing conditions. Use of this intermediate provides advantageouslyhigh purity product at expected yield.

In another embodiment the present disclosure provides lasmiditanacetate, which can be represented by the formula:

In another embodiment the invention provides lasmiditan acetate incrystalline form, and further provides lasmiditan acetate in crystallineform characterized by an X-ray powder diffraction pattern using CuKαradiation having an intense peak at diffraction angle 2-theta of 26.2°in combination with one or more of the peaks selected from the groupconsisting of 20.4°, 14.0°, and 17.9° (±0.2° respectively). In anotherembodiment the present invention provides a pharmaceutical compositioncomprising lasmiditan acetate according to the above embodiments withone or more pharmaceutically acceptable carriers, diluents, orexcipients. Preferably the pharmaceutical composition comprises aceticacid. Preferably the pharmaceutical composition comprises acetic acidand is for subcutaneous administration.

In another embodiment the invention provides a method of treatingmigraine in a patient comprising administering to a patient in need ofsuch treatment an effective amount of lasmiditan acetate. In anotherembodiment the invention provides a method of treating migraine in apatient comprising administering to a patient in need of such treatmentan effective amount of lasmiditan acetate with one or morepharmaceutically acceptable carriers, diluents, or excipients. Inanother embodiment the invention provides a method of treating migrainein a patient comprising administering to a patient in need of suchtreatment an effective amount of lasmiditan acetate with one or morepharmaceutically acceptable carriers, diluents, or excipients whereinthe composition comprises acetic acid.

In another embodiment the invention provides lasmiditan acetate for usein therapy. In another embodiment the invention provides apharmaceutical composition of lasmiditan acetate with one or morepharmaceutically acceptable carriers, diluents, or excipients for use intherapy. In another embodiment the invention provides a pharmaceuticalcomposition of lasmiditan acetate with one or more pharmaceuticallyacceptable carriers, diluents, or excipients, wherein the compositioncomprises acetic acid for use in therapy.

In another embodiment the invention provides lasmiditan acetate for usein the treatment of migraine. In another embodiment the inventionprovides a pharmaceutical composition of lasmiditan acetate with one ormore pharmaceutically acceptable carriers, diluents, or excipients foruse in the treatment of migraine. In another embodiment the inventionprovides a pharmaceutical composition of lasmiditan acetate with one ormore pharmaceutically acceptable carriers, diluents, or excipients,wherein the composition comprises acetic acid for use in the treatmentof migraine.

In another embodiment the present disclosure provides lasmiditanacetate, and pharmaceutical compositions comprising a high concentrationof lasmiditan acetate, e.g., about 10-200 mg/ml free base equivalent, inan aqueous carrier. In embodiments, the pharmaceutical compositioncomprises about 10-200 mg/ml free base equivalent lasmiditan in abuffered aqueous solution. In embodiments, the buffered aqueous solutionis at a pH of between pH 6.0-7.5 at 37° C. In embodiments, the bufferedaqueous solution comprises acetic acid.

In addition to an aqueous carrier, preferably sterile, deionized,distilled water, the pharmaceutical compositions described herein mayfurther comprise one or more pharmaceutically acceptable excipients orcosolvents. The term “pharmaceutically acceptable” refers to excipientsand cosolvents which are suitable for use in contact with the tissues ofhuman beings without excessive toxicity, irritation, allergic response,or other problem or complication, commensurate with a reasonablebenefit/risk ratio. Pharmaceutical compositions and processes forpreparing the same are well known in the art (see, e.g., Remington: TheScience and Practice of Pharmacy (A. Gennaro, et al., eds., 21st ed.,Mack Publishing Co., 2005)).

A pharmaceutical composition of lasmiditan acetate can be provided inbulk or in dosage unit form. It is especially advantageous to formulatepharmaceutical compositions of lasmiditan acetate in dosage unit formfor ease of administration and uniformity of dosage. The term “dosageunit form” as used herein refers to physically discrete units suitableas unitary dosages for the subject to be treated; each unit containing apredetermined quantity of active compound lasmiditan calculated toproduce the desired therapeutic effect in association with the requiredpharmaceutical carrier. A dosage unit form can be, e.g., an ampoule, avial, or a syringe.

In embodiments, the disclosure provides a pharmaceutical compositioncomprising an amount of lasmiditan acetate as described herein whereinthe amount is from 10 mg to 200 mg per dose. In embodiments, thedisclosure provides a pharmaceutical composition comprising an amount oflasmiditan acetate as described herein wherein the amount is 10 mg, 20mg, 30 mg, 40 mg, 50 mg, 100 mg, or 200 mg per dose. The forgoing dosesare based on an adult human of average weight. Smaller doses would beacceptable for individuals of lighter weight, for example the elderly orchildren. Therefore, in embodiments, the pharmaceutical composition maycomprise a smaller dose, such as 5, 10, or 15 mg.

As described herein, the highly concentrated aqueous solutions oflasmiditan acetate enables the administration of a singletherapeutically effective dose by injection of a high concentrationaqueous solution of lasmiditan, for example, by an intravenous,subcutaneous, or intramuscular route.

In embodiments, the disclosure provides high concentration aqueoussolutions of lasmiditan acetate. In embodiments, the high concentrationaqueous solution of lasmiditan acetate is formulated as a parenteraldosage form. In embodiments, the high concentration aqueous solutioncontains 10-200 mg/ml free base equivalent of lasmiditan. Inembodiments, the high concentration aqueous solution of lasmiditanacetate is in the form of a parenteral dosage form. In embodiments, theparenteral dosage form is a buffered aqueous solution of 10-200 mg/mlfree base equivalent of lasmiditan. In embodiments, the parenteraldosage form is a buffered aqueous solution of 10, 20, 30, 40, 50, 100 or200 mg/ml free base equivalent of lasmiditan. In embodiments, theparenteral dosage form is suitable for subcutaneous or intramuscularinjection. Preferably the parenteral dosage form is for subcutaneousinjection. In embodiments, the pH of the buffered solution is between pH6.0-7.5 at 37° C.

In embodiments, the buffered aqueous solution comprises a bufferingsystem based on an organic acid. In embodiments, the organic acid is adi- or tri-carboxylic acid. In embodiments, the di- or tri-carboxylicacid is selected from the group consisting of acetic acid and citricacid. In embodiments, the organic acid is succinic acid. In embodiments,the buffer is an acetic acid buffer. In embodiments, the bufferedaqueous solution is free of organic solvents. In embodiments, thebuffered aqueous solution is free of organic solvents and surfactants.In a preferred embodiment the buffered aqueous solution compriseslasmiditan acetate, and acetic acid, and sodium hydroxide, adjusted topH between 6.0-7.5 at 37° C.

In embodiments, the parenteral dosage form of lasmiditan acetate isprovided in the form of a pre-filled syringe suitable for administrationby a subcutaneous route. In embodiments, the pre-filled syringecomprises 10-50 mg/ml free base equivalent of lasmiditan. Inembodiments, the pre-filled syringe comprises 10, 20, 30, 40, 50 or 100mg/ml free base equivalent of lasmiditan. In embodiments, the lasmiditanis provided in a buffered aqueous solution having a pH 6.0-7.5 at 37° C.In embodiments, the pre-filled syringe is suitable for at-home use, forexample, for those migraine sufferers who might face an extreme andrapid onset of headache. In embodiments, the pre-filled syringe iscontained in a package with instructions for parenteral administration,preferably by subcutaneous injection. In embodiments, the pre-filledsyringe is in the form of an autoinjector with instructions forsubcutaneous injection.

In embodiments, the parenteral dosage form of lasmiditan acetate isprovided in the form of a vial containing 10-50 mg/ml free baseequivalent of lasmiditan. In embodiments, the parenteral dosage form oflasmiditan acetate is provided in the form of a vial containing 10, 20,30, 40, 50 or 100 mg/ml free base equivalent of lasmiditan. Inembodiments, the lasmiditan is provided in a buffered aqueous solutionhaving a pH 6.0-7.5 at 37° C.

The disclosure also provides methods for acute treatment of migraineheadache attacks, the methods comprising administering a therapeuticallyeffective dose of lasmiditan acetate as described herein. Inembodiments, the parenteral solution is administered by subcutaneousinjection. In embodiments, the parenteral solution comprises 10-50 mg/mlfree base equivalent of lasmiditan acetate in a buffered aqueoussolution at pH 6.0-7.5 at 37° C. In embodiments, the parenteral solutioncomprises 10, 20, 30, 40, 50 or 100 mg/ml free base equivalent oflasmiditan. In embodiments, the methods comprise administering a singletherapeutically effective dose of lasmiditan acetate in a volume of lessthan or equal to 1 ml, such as from about 0.5 to 1 ml, for example by asingle subcutaneous injection. In embodiments, the injection volume isabout 1 ml. In embodiments, the injection volume is about 0.5 ml.

The present invention provides a method for the treatment of migraine,in a patient in need thereof, comprising administering to the patient20-200 mg per subcutaneous dose of lasmiditan acetate and apharmaceutically acceptable diluent or carrier. The present inventionprovides a method for treatment of migraine, in a patient in needthereof, comprising administering to the patient 20 mg per subcutaneousdose of lasmiditan acetate and a pharmaceutically acceptable diluent orcarrier. The present invention provides a method for the treatment ofmigraine, in a patient in need thereof, comprising administering to thepatient 50 mg per subcutaneous dose of lasmiditan acetate and apharmaceutically acceptable diluent or carrier. The present inventionprovides a method for the treatment of migraine, in a patient in needthereof, comprising administering to the patient 75 mg per subcutaneousdose of lasmiditan acetate and a pharmaceutically acceptable diluent orcarrier. The present invention provides a method for the treatment ofmigraine, in a patient in need thereof, comprising administering to thepatient 100 mg per subcutaneous dose of lasmiditan acetate and apharmaceutically acceptable diluent or carrier. The present inventionprovides a method for the treatment of migraine, in a patient in needthereof, comprising administering to the patient 150 mg per subcutaneousdose of lasmiditan acetate and a pharmaceutically acceptable diluent orcarrier. The present invention provides a method for the treatment ofmigraine, in a patient in need thereof, comprising administering to thepatient 200 mg per subcutaneous dose of lasmiditan acetate and apharmaceutically acceptable diluent or carrier.

In some embodiments, a patient is a human who has been diagnosed ashaving a condition or disorder in need of prevention with apharmaceutical composition described herein. In some embodiments, apatient is a human that is characterized as being at risk of a conditionor disorder for which administration with a pharmaceutical compositiondescribed herein is indicated. In those instances where the disorderswhich can be treated by the methods of the present invention are knownby established and accepted classifications, such as migraine, episodicheadache, chronic headache, chronic cluster headaches, and/or episodiccluster headaches, their classifications can be found in varioussources. For example, at present, the fourth edition of the Diagnosticand Statistical Manual of Mental Disorders (DSM-IVTM) (1994, AmericanPsychiatric Association, Washington, D.C.), provides a diagnostic toolfor identifying many of the disorders described herein. Also, theInternational Classification of Diseases, Tenth Revision (ICD-10),provides classifications for many of the disorders described herein. Theskilled artisan will recognize that there are alternative nomenclatures,nosologies, and classification systems for disorders described herein,including those as described in the DSM-IV and ICD-10, and thatterminology and classification systems evolve with medical scientificprogress. Migraine patients can further be diagnosed with migraine, withor without aura (1.1 and 1.2), as defined by International HeadacheSociety (IHS) International Classification of Headache Disorders, 3rdedition, (ICHD-3) beta version (The International Classification ofHeadache Disorders, 3rd edition (beta version), Cephalalgia 2013; 33:629-808). In some embodiments, the human patient has been diagnosed withepisodic migraine prior to receiving chronic administration oflasmiditan, preferably nightly, to prevent migraine. In someembodiments, the human patient has been diagnosed with chronic migraineprior to receiving the antibody. In some embodiments, the human patientexperiences auras with their migraine headaches. In some embodiments,the human patient does not experience auras with their migraineheadaches.

As used herein “migraine” includes but is not limited to migraineattacks. As used herein “migraine attack” refers to the followingdescription. Symptoms may overlap within various phases of a migraineattack and not all patients experience the same clinical manifestations.In the prodrome phase, the majority of patients have premonitorysymptoms that may precede the headache phase by up to 72 hours. Theseinclude changes in mood and activity, irritability, fatigue, foodcravings, repetitive yawning, stiff neck, and phonophobia. Thesesymptoms may endure well into the aura, headache, and even postdromephases. Some patients experience an aura phase, wherein about one-thirdof patients experience transient neurological deficits during attacks.The ICHD-3 defines aura as 1 or more transient, fully reversibleneurological deficits, of which at least 1 has to have a unilaterallocalization, that develops over 5 minutes or more, and of which eachdeficit lasts between 5 and 60 minutes. While a visual aura, which mayshow positive (fortification spectra), negative (scotoma), or bothphenomena, is found in over 90% of the cases, and the most commondeficit, sensory, motor, speech, brain stem, and retinal aura symptomsmay also occur. A transient wave of neuronal depolarization of thecortex is believed to be the pathophysiological brain mechanismunderlying the clinical phenomenon of migraine aura. In the headachephase, headache attacks which may last 4 to 72 hours are accompanied bynausea, photophobia and phonophobia, or both. The headache ischaracterized as unilateral, pulsating, of moderate or severe intensity,and aggravated by physical activity; two of these characteristicssuffice to fulfill the diagnostic criteria. In the postdrome phase,characteristic symptoms reflect those observed during the premonitoryphase. Typical postdrome symptoms include tiredness, difficulties inconcentrating, and neck stiffness. It remains unclear whether thesesymptoms initiate in the premonitory phase and persist throughout theheadache phase into the postdrome phase, if they may also initiateduring the headache phase, or even appear after the headache phase hasended.

A “migraine headache” as used herein refers to headache, with or withoutaura, of >30 minutes duration, with both of the following requiredfeatures (A and B): A) at least 2 of the following headachecharacteristics: 1) unilateral location, 2) pulsating quality, 3)moderate or severe pain intensity, and 4) aggravation by or causingavoidance of routine physical activity; AND B) during headache at leastone of the following: a) nausea and/or vomiting, and/or b) photophobiaand phonophobia. A “probable migraine headache” as used herein refers toa headache of greater than 30 minutes duration, with or without aura,but missing one of the migraine features in the International HeadacheSociety ICHD-3 definition.

The term “effective amount” or “therapeutically effective amount” meansan amount or dose of lasmiditan acetate in a pharmaceutical composition,such as a total amount administered in an administration, which uponsingle or multiple dose administration to the patient, provides thedesired pharmacological effect in the patient, for example an amountcapable of activating 5-HT_(1F) receptors. In a preferred embodiment,“effective amount” means an amount of lasmiditan acetate that upon acuteadministration is capable of rendering a patient migraine attack freefollowing administration. A “dose” refers to a predetermined quantity oflasmiditan acetate calculated to produce the desired therapeutic effectin a patient. As used herein “mg” refers to milligram. As used herein,doses described in mg, refer to the active pharmaceutical ingredientlasmiditan, as free-base equivalent by mass, for instance a “100 mg”dose, refers to 100 mg of the active pharmaceutical ingredientlasmiditan as free-base equivalent. As used herein, a given dose may beinterpreted to describe doses of about the indicated amount, in thatdoses which are up to 10 percent higher or lower than the indicated doseare likewise contemplated to provide useful regimens in a manner similarto the indicated dose.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 : Graphical representation of an ¹H NMR spectrum (400 MHz,DMSO-d₆) of lasmiditan acetate containing maleic acid (internalstandard).

DETAILED DESCRIPTION

The reactions described herein may be performed via standard techniquesknown to the skilled artisan by employing routine glassware or may beperformed on pilot and/or production scale in equipment designed forsuch transformations. Further, each of these reactions described may beexecuted via either a batch process, or where applicable, a flowreaction methodology. The term “batch process” as used herein refers toa process in which raw materials are combined in a reactor or vessel andproduct is removed at the end of the reaction.

Additionally, certain intermediates described in the followingpreparations may contain one or more nitrogen protecting groups. Thevariable protecting group may be the same or different in eachoccurrence depending on the particular reaction conditions and theparticular transformations to be performed. The protection anddeprotection conditions are well known to the skilled artisan and aredescribed in the literature (See for example “Greene's Protective Groupsin Organic Synthesis”, Fourth Edition, by Peter G. M. Wuts and TheodoraW. Greene, John Wiley and Sons, Inc. 2007).

The abbreviations listed below when used herein are defined as follows:“A” means angstrom or angstroms. “ACN” means acetonitrile. “AcOH” meansacetic acid. “Bn” means benzyl; “nBuLi” means n-butyllithium. “CAS No.”means Chemical Abstracts Registry number. “DCM” means dichloromethane.“DMF” means N,N-dimethylformamide. “DIPEA” means diisopropylethylamine.“DMSO” means dimethyl sulfoxide (perdeuterated [d₆] if used for NMR).“EtOAc” means ethyl acetate. “EtOH” means ethanol or ethyl alcohol.“HBTU” means (2-(1H-bezotriazole-1-yl)-1,1,3,3-tetramethyluroniumhexafluorophosphate. “HPLC” means high performance liquidchromatography. “HTRF” means homogeneous time-resolved fluorescence.“hr” or “h” means hour or hours. “IPA” means isopropyl alcohol. “IPC”means in-process control. “LAH” means lithium aluminum hydride. “LCMS”means liquid chromatography mass spectrometry. “LDA” means lithiumdiisopropylamide. “Me” as a substituent in a structural representationof a compound represents a methyl group. “MeOH” means methanol or methylalcohol. “min” means minutes. “MS” means mass spectrometry or massspectrum. “MTBE” means methy tert-butyl ether. “NMR” means nuclearmagnetic resonance. “NMT” means not more than. “OAc” means acetate.“psig” means pounds per square inch gauge. “PyBOP” means(benzotriazol-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate).“RT” means room temperature/ambient temperature. “sec” means second orseconds as a unit of time. “TBS-Cl” means tert-butyldimethylsilylchloride. “TEA” means triethylamine. “THF” means tetrahydrofuran. “tR”means retention time. “w/w” means weight to weight in a ratio.

Improved routes for the preparation of lasmiditan are provided below asRoutes I and/or II, and other additional methods as provided below.

“Pharmaceutically acceptable salts” or “a pharmaceutically acceptablesalt” refers to the relatively non-toxic, inorganic and organic salt orsalts of the compounds of the present invention. It will be understoodby the skilled artisan that compounds of the present invention arecapable of forming salts. Some compounds of the present inventioncontain basic heterocycles, and accordingly react with any of a numberof inorganic and organic acids to form pharmaceutically acceptable acidaddition salts. Such pharmaceutically acceptable acid addition salts andcommon methodology for preparing them are well known in the art. See,e.g., P. Stahl, et al., HANDBOOK OF PHARMACEUTICAL SALTS: PROPERTIES,SELECTION AND USE, (VCHA/Wiley-VCH, 2008); S. M. Berge, et al.,“Pharmaceutical Salts”, Journal of Pharmaceutical Sciences, Vol 66, No.1, January 1977.

“Process scale” synthesis refers to preparations of 500 mg to 1000 kg,or more of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehemisuccinate. Preferably “process scale” syntheses are performed underGood Manufacturing Process (GMP) or similar conditions required forcommercial production of pharmaceutical products for human consumption.Preferably, “process scale” in the processes of Route I and/or II above,refers to batches produced in at least 1 kilogram, and/or batchesproduced in at least 10 kilograms, and/or batches produced in at least100 kilograms.

Scheme 1 depicts a process scale synthesis of lasmiditan hemisuccinatecompound I. N-Methylation of commercially available piperidine4-carboxylic acid 1 may be accomplished under various reductiveconditions recognizable to the skilled artisan, specifically treatmentof the secondary amine with about 1.3 equivalents of formadehyde in anexcess of formic acid, to obtain the N-methylpiperidine 2. Formation ofdiethylamide 3 may be achieved using conventional amide couplingreagents such as benzotriazole, HBTU or PyBOP or by converting thecarboxylic acid to the acid chloride, using reagents well known in theart such as oxalyl chloride or thionyl chloride. More specifically,N-methylpiperidine-4-carboxylic acid 2 may be converted to the acidchloride by treatment with about 1.2 equivalents of thionyl chloride atabout 50° C. for 1 hr, at which time the reaction mixture may be cooledto about 0° C. and 1.5 equivalents diethylamine and 3 equivalentstrimethylamine added. The free base is stirred with HCl to obtaindiethylamide hydrate hydrochloride 3. One skilled in the art willrecognize that pyridyl ketone 4 may be obtained by treatment ofdiethylamide 3 with the lithiated bromopyridine 3 a. More specifically,(6-bromo-2-pyridyl)lithium may be formed by treating 2,6 dibromopyridinewith n-BuLi at about −58° C. Separately, piperidine-4-diethylamidehydrochloride hydrate 3 may be treated with about 2 equivalents NaOH andthe resulting free base added to the lithiated species at about −58° C.The resulting mixture may be treated with HBr to form pyridylbromidehydrobromide 4. Amination of pyridylbromide hydrobromide 4 may beachieved using transition metal catalysis well known to one skilled inthe art. More specifically, to pyridylbromide 4 may be added about 0.075equivalents of Cu₂O, about 28 equivalents NH₃ in ethylene glycol andstirred to about 80° C. The reaction may be cooled to RT, quenched withH₂O, washed with 20% aqueous NaOH, slurried with 20% HCl in IPA and asmall amount of H₂O, to obtain a aminopyridine dihydrate dihydrochloride5 as a crystalline solid. Pyridylbenzamide hydrochloride 6 may beprepared by treating the free base of aminopyriyl 5 with the acidchloride 5 a. More specifically, aminopyridine dehydrate dihydrochloride5 may be treated with 6% aqueous NaOH to furnish the free base.Separately, 2,4,6-trifluorobenzoic acid may be treated with thionylchloride at about 100° C. and the aforementioned freebase of 5, toprovide pyridylbenzamide hydrochloride 6. Hemisuccinate I may be createdby treating hydrochloride 6 with about 2 equivalents of NaHCO₃ followedby about 0.55 equivalents succinic acid to obtain lasmiditanhemisuccinate compound I.

Scheme 2 depicts the synthesis of(6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone dihydratehydrochloride 5. Amination of pyridylbromide hydrobromide 4 may beachieved as outlined in scheme 1 using transition metal catalysis wellknown to one skilled in the art. More specifically, to pyridylbromide 4may be added about 0.075 equivalents of Cu₂O, about 28 equivalents NH₃in ethylene glycol and stirred at about 80° C. The reaction may becooled to RT, quenched with H₂O, washed with 20% aqueous NaOH, slurriedwith 20% HCl in IPA and a small amount of H₂O, to obtain aminopyridinedihydrate hydrochloride 5.

Scheme 3 illustrates a modified process synthesis to lasmiditanhemisuccinate I. N-Methylation of commercially available piperidine4-carboxylic acid 1 may be accomplished under various reductiveconditions recognizable to the skilled artisan, specifically treatmentof the secondary amine with about 1.3 equivalents of formadehyde in anexcess of formic acid, to obtain the N-methylpiperidine 2. Formation ofdiethylamide 3 may be achieved using conventional amide couplingreagents such as benzotriazole, HBTU or PyBOP or by converting thecarboxylic acid to the acid chloride, using reagents well known in theart such as oxalyl chloride or thionyl chloride. More specifically,N-methylpiperidine-4-carboxylic acid 2 may be converted to the acidchloride by treatment with about 1.2 equivalents of thionyl chloride atabout 50° C. for 1 hr, at which time the reaction mixture may be cooledto about 0° C. and 1.5 equivalents diethylamine and 3 equivalentstrimethylamine added. The free base is stirred with HCl to obtaindiethylamide hydrate hydrochloride 3. One skilled in the art willrecognize that pyridyl ketone 4 may be obtained by treatment ofdiethylamide 3 with the lithiated bromopyridine 3 a. More specifically,(6-bromo-2-pyridyl)lithium may be formed by treating 2,6 dibromopyridinewith n-BuLi at about −58° C. Separately, piperidine-4-diethylamidehydrochloride hydrate 3 may be treated with about 2 equivalents NaOH andthe resulting free base added to the lithiated species at about −58° C.The resulting mixture may be treated with HBr to form pyridylbromidehydrobromide 4. Amination of pyridylbromide hydrobromide 4 to obtainamide 6 may be achieved using transition metal catalysis well known toone skilled in the art. Specifically, the pyridyl ketone 4 may be sprungto its corresponding free base form with a suitable mineral base andsubjected to Buchwald-type coupling conditions, as is well known in theliterature. More specifically, the free base of compound 4 may bestirred in a suitable aprotic solvent, such as toluene or xylene,containing a mixture of about 1-5 weight % water, about 1.1 equivalentscommercially available 2,4,6-trifluorbenzamide (CAS #82019-50-9), about1.5 equivalents of potassium carbonate, about 0.005 to about 0.015equivalents of a suitable palladium catalyst, such as palladium(II)acetate, and about 0.01 to 0.02 equivalents of a suitable phosphineligand compound, such as Xantphos, XPhos, or DPEPhos. The resultingmixture may be heated at about 70° C. for about 12-24 hr. The reactionmixture may be diluted with a suitable mixture of water and organicsolvent, such as DCM or EtOAc, and the organic layer may be treated withan appropriate palladium scavenger, such as thiourea-modified silicagel, for about 8-24 hr at about RT to about 65° C. The resulting mixturemay be cooled, filtered, treated with activated charcoal, filtered, andconcentrated under reduced pressure. The resulting residue may bedissolved in an appropriate alcoholic solvent, such as ethanol, andtreated slowly with a solution of about 0.5 equivalents of succinic aciddissolved in ethanol at about 55° C. The resulting mixture may be cooledto RT over about 10 hr, and the resulting slurry may be slurry-milled bytreatment under a series of thermal cycles of heating to 60° C. andcooling back to RT over 4 hr. The resulting solid may be collected byfiltration, dried at about 40° C. for about 4 hr, and optionally jetmilled, to obtain lasmiditan hemisuccinate I.

Experimental Procedures

The following preparations of process intermediates further illustratethe invention and represent typical syntheses of various compounds. Thereagents and starting materials are readily available or may be readilysynthesized by one of ordinary skill in the art. It should be understoodthat the Preparations and Examples are set forth by way of illustration,and that various modifications may be made by one of ordinary skill inthe art.

LC-ES/MS is performed on an AGTLENT® HPi 100 liquid chromatographysystem. Electrospray mass spectrometry measurements (acquired inpositive and/or negative mode) are performed on a Mass SelectiveDetector quadrupole mass spectrometer interfaced to the HP1100 HPLC.LC-MS conditions (low pH): column: PHENOMENEX® GEMINI® NX C18 2.1 mm×50mm, 3.0μ; gradient: 5-100% B in 3 min, then 100% B for 0.75 min columntemperature: 50° C.+/−10° C.; flow rate: 1.2 mL/min; Solvent A:deionized water with 0.1% HCOOH; Solvent B: ACN with 0.1% formic acid;wavelength 214 nm. Alternate LC-MS conditions (high pH): column: XTERRA®MS C18 columns 2.1×50 mm, 3.5 μm; gradient: 5% of solvent A for 0.25min, gradient from 5% to 100% of solvent B in 3 min and 100% of solventB for 0.5 min or 10% to 100% of solvent B in 3 min and at 100% ofsolvent B for 0.75 min; column temperature: 50° C.+/−10° C.; flow rate:1.2 mL/min; Solvent A: 10 mM NH₄HCO₃ pH 9; Solvent B: ACN; wavelength:214 nm.

NMR spectra are performed on a Bruker AVIII HD 400 or 500 MHz NMRSpectrometer, obtained as CDCl₃ or (CD₃)₂SO solutions reported in ppm,using residual solvent [CDCl₃, 7.26 ppm; (CD₃)₂SO, 2.05 ppm] asreference standard. When peak multiplicities are reported, the followingabbreviations may be used: s (singlet), d (doublet), t (triplet), q(quartet), m (multiplet), br-s (broad singlet), dd (doublet ofdoublets), dt (doublet of triplets). Coupling constants (J), whenreported, are reported in hertz (Hz).

Chloride analysis is performed on an ESA CORONA® Plus instrumentequipped with a CORONA® CAD® (charged aerosol detector)-HPLC, AcclaimTrinity P1 (100×3.0 mm, 3 um), mobile phase: 50 mM ammonium acetate, pH˜5 in ACN.

The compounds described herein can be prepared by general methods knownto the skilled artisan or by processes described herein. Suitablereaction conditions for the steps of these schemes are well known in theart and appropriate substitutions of solvents and co-reagents are withinthe skill of the art. Likewise, it will be appreciated by those skilledin the art that synthetic intermediates may be isolated and/or purifiedby various well-known techniques as needed or desired, and thatfrequently, it will be possible to use various intermediates directly insubsequent synthetic steps with little or no purification. Furthermore,the skilled artisan will appreciate that in some circumstances, theorder in which moieties are introduced is not critical.

Preparation 1 1-methylpiperidine-4-carboxylic acid hydrochloride

Scheme 1, step A: To a jacketed reactor is chargedpiperidine-4-carboxylic acid (10.0 g, 77.5 mmol) and deionized water (40mL). The mixture is heated to reflux (95-100° C.). Formic acid (13.9 g,302 mmol) is added over 30 min. A 37% aqueous solution of formaldehyde(8.1 g, 101 mmol) is added to the mixture dropwise over at least 30 min.Water (0.3 mL) is used as a line rinse into the reactor. The mixture isstirred for 4 hr at reflux (95-100° C.) and sampled by HPLC for IPCanalysis (NMT 0.5% of piperidine-4-carboxylic acid). If the amount ofpiperidine-4-carboxylic acid is above 0.5%, the mixture is stirred 2additional hr. If the specification is met, the solution is concentratedunder vacuum until ˜20 mL of residual volume remains and the residue iscooled to 45-50° C. To the cooled solution is charged 33% aqueous HCl(12.8 g, 116 mmol) over not less than 30 min. Water (0.3 mL) is used asa line rinse into the reactor. Water is distilled off under vacuum until˜20 mL of residual volume remains. To the concentrated solution at45-50° C. is charged ACN (42.4 mL) and the mixture is concentrated underatmospheric pressure until ˜40 mL of residual volume remains. To theconcentrated solution at 45-50° C. is charged ACN (20.4 mL) and themixture is concentrated under atmospheric pressure until ˜40 mL ofresidual volume remains. The dilution/concentration operations arerepeated until the in process control for water content by Karl-Fischeranalysis is NMT 0.2%; during these operations a slurry forms. To theslurry is charged ACN (10.2 mL) at 45-50° C. The slurry is cooled to 20°C. over 1 h and stirred for an additional 2 h. The resulting solid isisolated by filtration and the cake is rinsed with ACN (10.2 mL). Thewet cake is dried at 40° C. under nitrogen at atmospheric pressure togive the title compound (12.1 g, 87% yield). MS (m/z): 144 (M+H).

Preparation 2 N,N-diethyl-1-methyl-piperidine-4-carboxamide hydratehydrochloride

Scheme 1, step B: To a jacketed reactor is charged1-methylpiperidine-4-carboxylic acid hydrochloride (30.0 g, 167 mmol),chlorobenzene (240 mL) and DMF (0.61 g, 8.35 mmol) and the resultingmixture is heated to 50° C. To the hot suspension is charged thionylchloride (24.2 g, 200.4 mmol) over a 1 hr period. Chlorobenzene (13.5mL) is used as a line rinse into the reactor. The mixture is stirred for5 hr after the completion of the thionyl chloride addition. The solutionis then cooled to 0 to 10° C. A solution prepared from diethylamine(17.7 g, 12.5 mmol) and TEA (50.7 g, 25 mmol) is charged to the coldreaction mixture over a 3 hr period. Chlorobenzene (13.5 mL) is used asa line rinse into the reactor. The mixture is stirred for 2 hr after thecomplete addition of the amine mixture. The reaction is treated with 20weight % aqueous NaOH (180.3 g, 902 mmol) and stirred at RT for 2 hr.Water (3 mL) is used as a line rinse into the reactor. The mixture isallowed to settle for 2 hr and the aqueous phase is removed. Theremaining organic phase is placed under vacuum. The mixture is heated todistill away the residual amines as well as most of the chlorobenzene.The reactor is vented to atmospheric pressure using nitrogen afterapproximately ten volumes of distillate have been collected. Theremaining solution is cooled to between 10° C. to 30° C. THE (120 mL)and water (4.54 g, 252 mmol) are charged to the reactor. With thereaction mixture at RT, the desired product is precipitated by theaddition of 20 weight % aqueous HCl in isopropanol (30.4 g, 167 mmol).THE (5.4 mL) is used as a line rinse into the reactor. After thecomplete addition of HCl, the suspension is stirred for 2 hr at RT. Theresulting solid is collected by filtration and washed with THE (75.0mL). The collected solids are dried under vacuum for 16 hr at 40° C. togive the title compound (35.5 g, 84% yield). MS (m/z): 199 (M+H).

Preparation 3 (6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanonehydrobromide

Scheme 1, step C: A suspension ofN,N-diethyl-1-methyl-piperidine-4-carboxamide hydrate hydrochloride(21.5 g, 85.1 mmol) in MTBE (109 mL) is treated with a 20 weight %aqueous solution of NaOH (34.0 g, 170 mmol). A water rinse (1.94 mL) isused to complete the addition. The mixture is stirred at RT for 30 min,the phases are allowed to settle, and phases are separated. The aqueousphase is extracted with MTBE (43.7 mL) and the organic phases combined.The organic phase is dried by distillation at atmospheric pressure untilthe in process control for water content by Karl-Fischer analysis is<0.10 weight %. If the target analysis is not met, the reaction ischarged with MTBE (43.7 mL) and the distillation is repeated. Typicallythree distillations are required to reach the target analysis for water.In a separate reactor is charged a mixture of 2,6-dibromopyridine (30.2g, 128 mmol) and MTBE (105 mL) and is cooled to less than −58° C. To thecooled suspension is charged a 2.5M solution of n-BuLi in hexanes (51.3mL, 128 mmol) over a 2 hr period. A rinse of MTBE (4.5 mL) is used tocomplete the transfer. The mixture is aged while maintaining thetemperature at less than −58° C. for an additional 2 hr after completen-BuLi addition. After aging, the solution ofN,N-diethyl-1-methyl-piperidine-4-carboxamide hydrate hydrochloride inMTBE is added to the cold reaction over a 45 min period. A rinse of MTBE(13.5 mL) is used to complete the transfer. The mixture is aged for atleast 30 min after complete addition ofN,N-diethyl-1-methyl-piperidine-4-carboxamide hydrate hydrochloride inMTBE. After aging, the reaction is warmed to 0° C. over 1 hr. The coldreaction mixture is added to a 2.5 M aqueous solution of HCl (146 mL,366 mmol) at a rate to maintain the quench temperature at NMT 30° C. Arinse of MTBE (13.5 mL) is used to complete the transfer. The mixture isstirred for at least 30 min after the transfer is complete and thephases are allowed to settle. The phases are separated and the aqueousphase is retained. n-BuOH (54.8 mL) is added to the aqueous phase andthe mixture is treated with a 20 weight % aqueous solution of NaOH (59.5g, 298 mmol). A rinse of water (2.80 mL) is used to complete thetransfer. The mixture is stirred for at least 30 min and the phases areallowed to settle. The phases are separated and the organic phase isretained. The aqueous phase is extracted with n-BuOH (54.8 mL). Thecombined organic phases are dried by distillation under vacuum to obtainan in process control for water content by Karl-Fischer analysis of<0.20 weight %. If the target analysis is not met, n-BuOH (41.1 mL) ischarged and the distillation is repeated. Typically, two distillationsare required to reach the in process control target analysis. Theconcentrated solution is clarified by filtration and a rinse with n-BuOH(89.6 mL) is used to complete the transfer and rinse the filter. Theclarified solution is treated with a 48 weight % aqueous solution of HBr(9.91 mL, 87.7 mmol) over a 90 min period. A rinse of n-butanol (13.8mL) is used to complete the transfer. A check of the pH shows thereaction mixture has a pH ˜1. The mixture is dried by distillation atatmospheric pressure to obtain an in process control for water contentby Karl-Fischer analysis of <0.30 weight %. The mixture is concentratedto 172 mL. If the target analysis is not met, n-BuOH (54.8 mL) ischarged and the distillation is repeated. The mixture is cooled to 20°C. and stirred for 12 hr. The resulting solids are collected byfiltration and washed twice with n-BuOH (10.75 mL). The solids are driedunder vacuum at 60° C. to obtain the title compound (24.8 g, 80% yield).MS (m/z): 283, 285 (⁷⁹Br, ⁸¹Br, M+H).

Preparation 4 (6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanonedihydrate dihydrochloride

Scheme 1, step D: To a pressure reactor is charged(6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone hydrobromide (30 g,82.9 mmol) and Cu₂O (880 mg, 6.2 mmol). The headspace is exchanged withnitrogen/vacuum purge cycles three times. To the solids are charged asolution of NH₃/ethylene glycol (273.5 g total, 39.1 g NH₃, 2.33 mol;210 mL ethylene glycol) and the resulting mixture is stirred at RT for 2hr. The mixture is heated to 80° C., stirred for 10 h, and cooled to RTfor in process control sampling for(6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone hydrobromide NMT 2%.If the target analysis is not met, the reaction is stirred for another 4hr at 80° C. and sampled again. To the completed reaction is charged H₂O(90 mL) and the mixture is filtered. The filtrate is charged intoaqueous NaCl (253.9 g NaCl, 2.73 mol, 13.7 L/kg H₂O) and the resultingmixture is stirred at RT for 10 min. To the mixture is charged a 20%aqueous solution of NaOH (4.44 equiv., 368 mmol) and the biphasicmixture is stirred at RT for 5 min. The mixture is extracted with MTBE(90 mL) 4-5 times at RT. The combined MTBE layers are treated with 5weight % carbon for 30 min and the carbon is removed by filtration. Theorganic filtrate is concentrated to ˜150 mL under vacuum. To theconcentrated filtrate is charged IPA (200 mL) and the solution isconcentrated to ˜150 mL under vacuum two times. The IPA distillationsare repeated as needed to meet the target analyses for in processcontrol of water. Water content is confirmed to be not more than 0.2% byKarl-Fischer analysis. In a separate reactor is charged a 20 weight %solution of HCl in IPA (30 g, 166 mmol) and water (10.5 mL) at RT. Theconcentrated product mixture is charged to the HCl solution over 90 min.The resultant slurry is stirred at RT for not less than 8 hr. The slurryis filtered, rinsed twice with a mixture of 95:5 IPA/H₂O (36 mL) at RT,and dried under vacuum at 40° C. for 16-24 hr to afford the titlecompound (18.4 g 68% yield). MS (m/z): 220 (M+H). ¹H NMR (400 MHz,D2O/DMSO-d6) δ ppm 1.74-1.88 (m, 2H), 2.05 (br d, J=14.9 Hz, 2H), 2.73(s, 3H), 3.01 (td, J=13.1, 2.6 Hz, 2H), 3.41-3.50 (m, 2H), 3.55 (tt,J=12.0, 3.5 Hz, 1H), 7.14 (dd, J=9.0, 0.7 Hz, 1H), 7.59 (d, J=7.2 Hz,1H), 7.90 (dd, J=9.0, 7.2 Hz, 1H). ¹³C NMR (101 MHz, D2O//DMSO-d6) δ ppm27.4, 40.7, 44.6, 54.6, 117.2, 121.1, 137.9, 145.2, 156.2, 196.3.Chloride analysis: 20.23% (n=2).

X-Ray Powder Diffraction (XRPD) of Crystalline Forms

The XRPD patterns of crystalline solids are obtained on a Bruker D4Endeavor X-ray powder diffractometer, equipped with a CuKα source and aVantec detector, operating at 35 kV and 50 mA. The sample is scannedbetween 4 and 40 2θ°, with a step size of 0.008 2θ° and a scan rate of0.5 seconds/step, and using 1.0 mm divergence, 6.6 mm fixedanti-scatter, and 11.3 mm detector slits. The dry powder is packed on aquartz sample holder and a smooth surface is obtained using a glassslide. The crystal form diffraction patterns are collected at ambienttemperature and relative humidity. Crystal peak positions are determinedin MDI-Jade after whole pattern shifting based on an internal NIST 675standard with peaks at 8.853 and 26.774 2θ°. It is well known in thecrystallography art that, for any given crystal form, the relativeintensities of the diffraction peaks may vary due to preferredorientation resulting from factors such as crystal morphology and habit.Where the effects of preferred orientation are present, peak intensitiesare altered, but the characteristic peak positions of the polymorph areunchanged. See, e.g. The United States Pharmacopeia #23, NationalFormulary #18, pages 1843-1844, 1995. Furthermore, it is also well knownin the crystallography art that for any given crystal form the angularpeak positions may vary slightly. For example, peak positions can shiftdue to a variation in the temperature at which a sample is analyzed,sample displacement, or the presence or absence of an internal standard.In the present case, a peak position variability of ±0.2 2θ° is presumedto take into account these potential variations without hindering theunequivocal identification of the indicated crystal form. Confirmationof a crystal form may be made based on any unique combination ofdistinguishing peaks.

A sample of Preparation 4,(6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone dihydratedihydrochloride, is characterized by an XRD pattern using CuKα radiationas having diffraction peaks (20 values) as described in Table 1 below,and in particular having peaks at 8.3° in combination with one or moreof the peaks selected from the group consisting of 16.6°, 23.5°, and33.7°, with a tolerance for the diffraction angles of 0.2 degrees.

TABLE 1 X-ray powder diffraction peaks of the crystalline compound ofPreparation 4; (6-amino-2-pyridyl)-(1-methyl-4- piperidyl)methanonedihydrate dihydrochloride Relative Intensity (% of most intense PeakAngle (° θ) +/−0.2° peak) 1 8.3 100.0% 2 16.6 49.8% 3 19.9 8.1% 4 22.515.2% 5 23.5 25.7% 6 25.1 17.1% 7 28.8 11.0% 8 29.7 17.0% 9 30.0 13.9%10 33.7 23.7%

Preparation 52,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehydrochloride

Scheme 1, step E: To a suspension of(6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone dihydrochloridedihydrate (10 g, 30.6 mmol) in chlorobenzene (65 mL) is charged 6 w/w %aqueous NaOH (3 g, 75 mmol). The biphasic mixture is heated to 54° C.with stirring for 30 min, the mixture is allowed to separate over 30min, and the layers are separated at 54° C. The aqueous layer isback-extracted with chlorobenzene (45 mL) at RT. The organic layers arecombined and distilled under vacuum to ˜62 mL to afford a solution of(6-aminopyridin-2-yl)(1-methylpiperidin-4-yl)methanone. In a separatereactor is charged 2,4,6-trifluorobenzoic acid (5.9 g, 1.1eq, 33.7mmol), DMF (62 mg, 0.85 mmol) and chlorobenzene (32 mL) and the mixtureis heated to 80° C. To the heated mixture is charged thionyl chloride(4.37 g, 37 mmol) over 4 hr at 80° C. The mixture is stirred at 80° C.for at least 6 hr, and heated to 100° C. for at least 6 hr to purgeresidual HCl gas. The solution of acid chloride is cooled to RT andtransferred to a separate reactor. The acid chloride solution is heatedto 100° C. and charged with(6-aminopyridin-2-yl)(1-methylpiperidin-4-yl)methanone over 4 hr. Theresulting slurry is agitated for an additional 3 hr at 100° C. andcooled to RT. To the cooled slurry is charged ACN (100 mL). Theresulting slurry is heated to 80° C. for 1 hr and cooled to RT over 2hr. The resulting slurry is further agitated at RT for an additional 1hr and filtered. The filter cake is washed with ACN (10 mL) at RT. Thecollected solids are dried under vacuum at 100° C. for 16 hr to obtainthe title compound (10.7 g, 85% yield). MS m/z 378 (M+H).

Alternate Procedure for Preparation 5

To a suspension of (6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanonedihydrochloride dihydrate (10 g, 30.6 mmol) in chlorobenzene (65 mL) ischarged 6 w/w % aqueous NaOH (2.97 g, 74.4 mmol). The biphasic mixtureis heated to 54° C. with agitation for 30 minutes and the layers areallowed to separate over 30 min. The layers are separated at 54° C. Theaqueous layer is back-extracted with chlorobenzene (45 mL) at RT. Theorganic layers are combined and distilled under vacuum to ˜62 mL toafford a solution of(6-aminopyridin-2-yl)(1-methylpiperidin-4-yl)methanone. In a separatereactor is charged 2,4,6-trifluorobenzoic acid (5.9 g, 33.7 mmol), DMF(62 mg, 0.85 mmol) and chlorobenzene (32 mL) and the mixture is heatedto 80° C. To the heated mixture is charged thionyl chloride (4.4 g, 37mmol) over 4 hr at 80° C. The mixture is agitated at 80° C. for at least6 hours and heated to 100° C. for at least 6 hours to purge residual HClgas. The solution of acid chloride is cooled to RT and transferred to aseparate reactor. The acid chloride solution is heated to 100° C. and tothe solution is charged(6-aminopyridin-2-yl)(1-methylpiperidin-4-yl)methanone over 4 hr. Theresulting slurry is agitated for an additional 3 hours at 100° C. andcooled to RT. To the cooled slurry is charged ACN (100 mL). Theresulting slurry is heated to 80° C. for 1 hr and cooled to RT over 2hr. The resulting slurry is further agitated at RT for an additional 1hr and the resulting solids are collected by filtration. The filter cakeis washed with ACN (10 mL) at RT. The solids are dried under vacuum at100° C. for 16 hr to obtain the title compound (10.7 g, 85% yield). MSm/z 378 (M+H).

Preparation 62,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehemisuccinate

Scheme 1, step F: To a reactor is charged2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehydrochloride (20 g, 48.4 mmol) and MTBE (202 mL). To the stirred slurryat RT is charged a solution of aqueous NaHCO₃ (8.13 g, 96.8 mmol NaHCO₃in 200 mL water) over 1 hr. The biphasic mixture is separated and theaqueous layer is back-extracted with MTBE (202 mL). The combined organiclayers are distilled under vacuum to a final volume of ˜200 mL. To thedistilled solution is charged SiO₂ (2 g), the resulting mixture isstirred for 30 min at RT, filtered, and the filter cake is rinsed withMTBE (10.8 mL). To the filtrate is charged carbon (340 mg;alternatively, the solution can be filtered through a carbon cartridge)and the resulting mixture is stirred at RT for 30 min and filteredthrough a 1-5 m filter, followed by a rinse with MTBE (21.6 mL). Thefiltrate is distilled under vacuum to˜80 mL. To the concentratedsolution is charged ethanol (114 mL) and the resulting solution isdistilled under vacuum to˜84 mL. The EtOH add-backs and distillationsare continued until the water content is not more than 1% byKarl-Fischer analysis, providing a dry solution of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide.In a separate reactor is charged succinic acid (3.03 g, 25.7 mmol) andEtOH (60 mL). The mixture is stirred and heated at 33° C. until thesuccinic acid is dissolved completely.

The solution of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamideis heated to 55° C. To the heated solution is charged a portion of thesolution of succinic acid in EtOH (roughly 1.0 L/kg). The resultingsolution is then seeded by the addition of 1 weight % of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehemisuccinate (200 mg). The resulting slurry is stirred for 30 min andthe remainder of the succinic acid in EtOH solution is charged at aconstant rate over 2 hr. The reactor contents are stirred for 30 min andcooled to RT, linearly over 2.5 hr. The resulting slurry is stirred atRT for not less than 3 hr. The slurry is filtered and the collectedsolids are washed with EtOH (60 mL). The solids are dried under vacuumat 45° C. for 16 hr to obtain the title compound (20.4 g, 85% yield). MSm/z 378 (M+H). ¹H NMR (500 MHz, DMSO-d₆) δ ppm 1.59 (d, J=12.1 Hz, 2H),1.84 (d, J=12.8 Hz, 2H), 2.08 (t, J=11.4 Hz, 1H), 2.38 (s, 2H), 2.24 (s,3H), 2.89 (d, J=13.1 Hz, 2H), 3.70 (s, 1H), 7.40 (dd, J=9.4, 7.8 Hz,2H), 7.75 (d, J=7.6 Hz, 1H), 8.08 (t, J=7.9 Hz, 1H), 8.39 (d, J=8.2 Hz,1H), 11.47 (s, 1H).

Alternative Procedure for Preparation 6

Scheme 3, Steps D, E and F: To a jacketed reactor is charged(6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone (50 g, 137 mmol) andtoluene (400 mL). Water is added (250 mL), followed by KOH pellets (13.6g, 206 mmol) and the mixture is stirred for 3 hr at RT. The contents ofthe reactor are filtered and returned to the reactor. The aqueous layeris drained and if necessary, the organic layer is treated with activatedcarbon to remove color. The mixture is concentrated at 50° C. andreduced pressure to 150 mL. Toluene (225 mL) is added back to thereactor under a nitrogen atmosphere and K₂CO₃ (28.5 g, 206 mmol),2,4,6-trifluorobenzamide (26.5 g, 151 mmol), and water (2.5 mL) areadded, and the contents are stirred at RT. To a separate flask under anitrogen atmosphere is charged toluene (20 mL), Pd(OAc)₂ (154 mg, 0.68mmol), and Xantphos (795 mg, 1.37 mmol), and the contents are stirred atRT for 30 minutes. The resulting solution is transferred to the reactorand the reactor is heated to 70° C. with stirring. After 5 hr, themixture is sampled for IPC HPLC analysis of NMT 0.1%(6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone. If the amount of(6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone is not met, themixture is stirred 5 additional hr and sampled again. If the IPC is met,the mixture is stirred for an additional 12 hours at 70° C. The contentsof the reactor are then cooled to 45° C. Water (250 mL) and EtOAc (250mL) are added and the mixture is stirred for 1 hr. The agitation isstopped, and the layers are allowed to separate. The aqueous layer isremoved and discarded. Water (250 mL) is charged and the resultingmixture is stirred for 1 hr. Agitation is stopped and the layers areallowed to separate. The aqueous layer is removed and discarded.Thiourea-modified silica gel (5 g) is charged and the reactor is heatedto 60° C. for 8 hr with stirring. The contents of the reactor are cooledto RT. The solution is filtered and returned to the reactor. Thethiourea-modified silica gel filter cake is rinsed with EtOAc (150 mL)and the rinse is returned to the reactor. If necessary, an activatedcarbon treatment may be implemented to remove color. The solution ispassed through a polish filter to obtain a solution of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide.

The solution of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamideis concentrated at 50° C. under reduced pressure to ˜150 mL. EtOH (250mL, denatured with toluene) is charged and the contents are concentratedat 50° C. under reduced pressure to ˜150 mL. This is repeated for atotal of 3 cycles to achieve adequate toluene removal before a finaltoluene charge to a total of 250 mL. The resulting toluene mixture isheated to 55° C. To a separate vessel is charged succinic acid (8.6 g,73 mmol) and EtOH (200 mL, denatured with toluene). The contents of thevessel are stirred until complete dissolution of succinic acid isachieved. Approximately 30 mL of the succinic acid solution istransferred to the solution of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamideand the resulting solution is stirred at 55° C.2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehemisuccinate is added as seed crystals either as a solid or a slurry intoluene denatured EtOH. The remainder of the succinic acid in toluenedenatured EtOH solution is transferred to the reactor over 1.5 hr. Thecontents of the reactor are cooled to RT over 10 hours. The resultingslurry may be slurry milled to control particle size. If slurry milled,the contents of the reactor may undergo a series of thermal cycles viaheating to 60° C. and cooling back to RT over 4 hr with stirring tofurther control particle size distribution. The slurry is filtered andrinsed with EtOH (100 mL, denatured with toluene) and dried at 40° C.under reduced pressure for 12 hr to provide the title compound (43.9 g,73% yield). MS m/z 378 (M+H). The dried solids may then be jet milledfor further particle size control.

Description of Drug Product Methods of Manufacture

In an embodiment lasmiditan prepared by the processes provided hereincan further be prepared as certain useful drug product forms. In anembodiment such drug product forms are available as oval 50 and 100 mg,debossed, aqueous film-coated, immediate-release tablets. The 50 mgtablet is a light gray, oval tablet debossed with “4312” on one side and“L-50” on the other. The 100 mg tablet is light purple, oval tabletdebossed with “4491” on one side and “L-100” on the other.

The following unit formula can be used in manufacturing lasmiditantablets. Ingredient naming conventions are according to the USP.

TABLE 2 Unit Formula for Lasmiditan 50 mg and 100 mg Tablets QuantityComponent 50 mg 100 mg Description Core Tablet - IntragranularComponents Lasmiditan Hemisuccinate^(a) 57.824 115.65 Active IngredientLasmiditan Free Base 50 100 Microcrystalline Cellulose^(a) 30.86 61.71Filler Pregelatinized Starch 7.500 15.00 Binder Croscarmellose Sodium5.630 11.26 Disintegrant Sodium Lauryl Sulfate, 0.5600 1.120 WettingAgent Purified Water^(b) — — Granulating Liquid (4.774)^(c) (9.548)^(c)Core Tablet - Extragranular Components Croscarmellose Sodium 7.880 15.76Disintegrant Magnesium Stearate 2.250 4.500 Lubricant Core TabletWeight: 117.3 234.5 Film Coating Color Mixture Gray 3.519 — ColorantColor Mixture Purple — 7.035 Colorant Purified Water^(d) — — SolventTotal Tablet Weight: 120.8 241.5 Table 2 Notes: ^(a)A salt conversionfactor of 0.86469 is used to calculate the quantity of lasmiditanhemisuccinate. The quantity of microcrystalline cellulose may beadjusted accordingly to maintain target tablet weight. ^(b)PurifiedWater is used in the granulation operation. The majority of the water issubsequently removed during the drying operation. ^(c)A small quantityof residual water remains following the drying process, which may be inthe form of free water or as water of hydration associated with drugsubstance. ^(d)Purified water is used in the coating unit operation. Thecoating suspension is comprised of 20% w/w solids. Sufficient coating issprayed to target a weight gain of 3%. This water is removed during thecoating unit operation.

Tablet Manufacture:

Lasmiditan tablets are manufactured using a high shear wet granulationprocess which is described as follows. High Shear Wet Granulation:Sodium lauryl sulfate is passed through a security screen and added topurified water to form the granulating liquid. Lasmiditan drug substanceand the excipients to be wet granulated (microcrystalline cellulose,pregelatinized starch, croscarmellose sodium) are passed through asecurity screen and combined in the granulator. The materials are mixedwith the main impeller of the granulator prior to the addition of thegranulating liquid. The powder blend is granulated in the granulator byadding the granulating liquid, while the powder is mixing. Uponcompletion of the liquid addition, the granulation is wet massed tofacilitate liquid distribution. The granulation is coarsely sized bypassing through a cone mill prior to drying.

Fluidized Bed Drying: The granulation is dried in a fluidized bed dryeruntil a moisture value of (50 mg and 100 mg: NMT 7%) is achieved, asmeasured by a gravimetric loss on drying method, or using ascientifically justified equivalent method. The dried granules arepassed through a cone mill and added to a tumble bin.

Final Blend—Extragranular Powder Blend and Final Blend Lubrication: Theextragranular croscarmellose sodium is passed through a security screen,and added to the dry milled granules in the tumble bin. The materialsare tumble blended. The extragranular magnesium stearate is passedthrough a security screen, and added to the tumble bin. The materialsare tumble blended.

Tablet Compaction: The blended granulation is compressed into tabletsusing a rotary compression machine. The compression parameters areselected at the start of a batch to achieve the target average (n=10)tablet weight of (50 mg: 111.4 mg-123.2 mg, 100 mg: 222.8-246.2 mg) andaverage (n=10) tablet breaking force of (50 mg: 4.1-13.7 kiloponds, 100mg: 6.0-17.9 kiloponds). The average (n=10) core tablet weight, tabletbreaking force, and tablet thickness are evaluated during start-up andthroughout the compression unit operation. Tablet friability and tabletdisintegration time are evaluated at the start-up of compression.

Film Coating of Core Tablets: The color mixture (gray for the 50 mg, andpurple for the 100 mg) is passed through a security screen and mixedwith purified water to form the coating suspension. The tablets arefilm-coated with the suspension utilizing spray guns in a perforatedcoating pan. The pan is rotated while the coating suspension is appliedat a controlled rate with pneumatic atomization, and drying air ispassed through the tablet bed to yield an acceptable exhausttemperature. Sufficient coating is sprayed to achieve the desiredpercent coating applied (50 mg and 100 mg: 2.0%-5.5%). The film-coatedtablets are inspected for visual quality following the completion ofcoating step. The film-coated tablets are discharged into bulk storagecontainers and may be sorted (optional).

Container Closure System—Unit-Dose Blisters:

Lasmiditan tablets are provided in individual blister cavities formedfrom polychlorotrifluoroethylene (PCTFE)/polyvinylchloride (PVC)laminated film and sealed with aluminum foil laminate lidding materialwhich contains a PVC-based heat seal coating.

Preparation and Description of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamideacetate

Preparation of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamideacetate (also referred to as lasmiditan acetate) is performed by placing2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide(541 mg, 1.6 mmol) in isopropyl acetate (5 mL) while stirring at 1000rpm at room temperature. Acetic acid (100 μL) is added. A white solidprecipitates out of solution after about two minutes of stirring.Stirring is shut off after 10 minutes, the white solid is collected byvacuum filtration on Whatman paper and dried in place under air streamfor 10 minutes to yield the title compound (650 mg, 92% yield).

Counterion stoichiometry is measured by nuclear magnetic resonance usingan Agilent 400-MHz spectrometer. A sample solution is prepared bydissolving the prepared2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamideacetate (7.715 mg) and maleic acid (5.949 mg), used as standard forother measurements, in DMSO-d₆ (approximately 0.75 mL). A ¹³C-decoupled¹H spectrum of the sample from 0-12 ppm is acquired using the followingparameters: 90-degree excitation pulse, 64 scans, 25-second relaxationdelay, and 4.5-second acquisition time. The resonances for acetate atapproximately 1.9 ppm (3H) and for2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamideat approximately 7.8 ppm (1H) are integrated to obtain areas of 29094and 9508, respectively. The molar ratio of acetate to2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamideis calculated by taking the ratio of these areas, accounting for thedifference in proton count for resonance, yielding an observed molarratio of 29094/(3×9508)=1.02 acetate:2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide.See for example FIG. 1 which shows an ¹H NMR spectrum (400 MHz, DMSO-d₆)of lasmiditan acetate containing maleic acid (internal standard). Thisresult provides experimental evidence that the prepared example of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamideacetate is a mono-acetate salt.

X-Ray Powder Diffraction (XRPD) of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamideacetate

The XRPD patterns of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamideacetate crystalline solids are obtained on a Bruker D4 Endeavor X-raypowder diffractometer, equipped with a CuKα source and a Vantecdetector, operating at 35 kV and 50 mA. The sample is scanned between 4and 40 2θ°, with a step size of 0.008 2θ° and a scan rate of 0.5seconds/step, and using 1.0 mm divergence, 6.6 mm fixed anti-scatter,and 11.3 mm detector slits. The dry powder is packed on a quartz sampleholder and a smooth surface is obtained using a glass slide. The crystalform diffraction patterns are collected at ambient temperature andrelative humidity. Crystal peak positions are determined in MDI-Jadeafter whole pattern shifting based on an internal NIST 675 standard withpeaks at 8.853 and 26.774 2θ°. It is well known in the crystallographyart that, for any given crystal form, the relative intensities of thediffraction peaks may vary due to preferred orientation resulting fromfactors such as crystal morphology and habit. Where the effects ofpreferred orientation are present, peak intensities are altered, but thecharacteristic peak positions of the polymorph are unchanged. See, e.g.The United States Pharmacopeia #23, National Formulary #18, pages1843-1844, 1995. Furthermore, it is also well known in thecrystallography art that for any given crystal form the angular peakpositions may vary slightly. For example, peak positions can shift dueto a variation in the temperature at which a sample is analyzed, sampledisplacement, or the presence or absence of an internal standard. In thepresent case, a peak position variability of ±0.2 2θ° is presumed totake into account these potential variations without hindering theunequivocal identification of the indicated crystal form. Confirmationof a crystal form may be made based on any unique combination ofdistinguishing peaks.

A prepared sample of crystalline acetate salt is characterized by anXRPD pattern using CuKα radiation as having diffraction peaks (2-thetavalues) as described in Table 3 below, and in particular having peaks at26.2 in combination with one or more of the peaks selected from thegroup consisting of 20.4, 14.0, and 17.9; with a tolerance for thediffraction angles of 0.2 degrees.

TABLE 3 X-ray powder diffraction peaks of crystalline2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide acetate salt2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide acetate Angle (°2-Theta) +/− Relative Intensity (% ofmost Peak 0.2° intense peak) 1 8.2 40.4% 2 9.7 29.6% 3 12.0 35.8% 4 14.065.0% 5 16.9 43.8% 6 17.9 63.2% 7 20.4 70.5% 8 23.3 30.6% 9 24.3 43.9%10 26.2 100.0%

Solubility of Lasmiditan Acetate and Use for Formulations forSubcutaneous Injection

Lasmiditan acetate was discovered to be surprisingly superior to manyalternative salt forms for the preparation of a subcutaneous formulationto deliver a dose of lasmiditan in minimal volume in near-physiologicalfluid. Lasmiditan acetate was discovered to enable a desired dose targetof about 50 mg in the minimal volume of less than or equal to about 1mL, while at the same time achieving a desired target pH being close toneutral, and in addition being relatively isotonic and physically andchemically stable. Using lasmiditan hemisuccinate it was experimentallydetermined that achieving >50 mg/mL solubility, at close to neutral pH,was difficult without using co-solvents. For solubility determinations 0mmol buffer is made with a respective acid and salt, and pH is adjustedby changing the acid/salt ratio. Excess solid is equilibrated at RTovernight, and solution concentration is analyzed by TPLC and solid wascharacterized by XRPD. In contrast, for lasmiditan acetate it wasdiscovered that solubility of >>0 mg/mL can be achieved, at close toneutral pH, without adjusting the pH.

TABLE 4 Solubility of Lasmiditan Acetate in acetate, citrate, orphosphate buffer. A 10 mmol buffer is made with respective acid andsalt, and pH is adjusted by changing acid/salt ratio. All added solutewent into solution to form a viscous solution. Solubility Buffer pH pH(Free Base eq. media Solid form (before) (after) mg/mL) AcetateLasmiditan Acetate 3.69 6.25 >441 Acetate Lasmiditan Acetate 4.056.41 >430 Acetate Lasmiditan Acetate 4.4 6.31 >412 Acetate LasmiditanAcetate 5.09 6.50 >430 Acetate Lasmiditan Acetate 5.71 6.56 >486 CitrateLasmiditan Acetate 2.5 5.60 >300 Citrate Lasmiditan Acetate 3.845.72 >300 Citrate Lasmiditan Acetate 4.99 6.23 >300 Citrate LasmiditanAcetate 5.96 6.24 >300 Phosphate Lasmiditan Acetate 8.24 6.42 >300

It was discovered that lasmiditan acetate surprisingly demonstrates ahighly advantageous combination of pharmaceutical properties. Lasmiditanacetate enables the desired solubility to provide high concentrationformulations having a less than or equal to about 1 mL dose volume, forthe desired unit doses, which is critical for clinical applications suchas use in available autoinjector devices. In addition, dissolution oflasmiditan acetate at 50 mg/mL results in close to neutral pH (pHapproximately 6.8), is isotonic, and stable for at least 2 months.Lasmiditan acetate demonstrates significantly higher solubility thanlasmiditan hemisuccinate salt with a desirable pH profile and enablesdelivery of the required unit doses in volumes of about 1 mL or less.

These results indicate that lasmiditan acetate enables a surprisinglyhigh concentration aqueous solution of lasmiditan, with usefulpharmaceutical properties for clinical parenteral administration, suchas subcutaneous injection. The pharmacological activities of lasmiditanare well-established (Curto, M. et al. Profiling lasmiditan as atreatment option for migraine. Expert Opinion on Pharmacotherapy (2020),Volume 21, Issue 2, pages 147-153). Preferably subcutaneous injection isadministered by prefilled syringe or autoinjector, employing devicesknown to the skilled artisan (See e.g. Stauffer V L, et al., Comparisonbetween prefilled syringe and autoinjector devices on patient-reportedexperiences and pharmacokinetics in galcanezumab studies., PatientPrefer Adherence. (2018) 12:1785-1795, and van den Bemt B J F, et al., Aportfolio of biologic self-injection devices in rheumatology: howpatient involvement in device design can improve treatment experience.,Drug Deliv. (2019), 26(1):384-392). These formats provide for a fixeddose, with no measurement by patient, providing dose accuracy andsafety, while enabling self-reliance by the patient. Use of thelasmiditan acetate salt for acute treatment of migraine attack informats such as autoinjectors provide improved tools for institutionalpatients, such as those in hospital emergency settings where patient useof tablets is impaired by the migraine attack and associated nausea andvomiting, and the patients and/or providers prefer an improvedinjectable form of lasmiditan. Lasmiditan acetate parenteralformulations are expected to provide immediate release, enabling rapidtime to onset of action, and may preferably allow for shorter time toefficacy relative to oral dose forms when used on demand at the outsetof a migraine attack.

Providing lasmiditan formulations for injection at neutral andphysiological pH (approximately 6.0-7.5), and isotonic withphysiological fluid (e.g. 280 to 300 mosm/kg), is clinically highlydesirable and considered to minimize the likelihood of pain oninjection, and/or tissue irritation, for example. Achieving injectionvolumes of about 1 ml or less enable the use of available injectortechnologies, such as autoinjectors, and provide for improved injectionand delivery experiences for patients with regard to injection time,and/or pain on injection, for example. Enabling the use of pre-filledsyringes, pens, and/or autoinjector technologies is clinicallysignificant for migraine patients as these devices provide an ease ofuse during migraine attacks when patients are often under duress at thetime of product use. Further, enabling the use of pre-filled syringes,pens, and/or autoinjector technologies is clinically significant becausethey provide portable access to medication, at any time, which is easilyaccessible in the course of daily living, where a migraine attack canoccur at any time.

The following unit formula can be used in manufacturing lasmiditansolution for injection.

TABLE 5 Unit Formula for Lasmiditan 50 mg Solution in an AutoinjectorQuantity Component (mg/autoinjector) Description Lasmiditan 50.00 ActiveIngredient Lasmiditan Acetate 57.96 Acetic Acid Solution (10%)Sufficient to pH Adjustment Adjust pH if needed Sodium HydroxideSufficient to pH Adjustment Solution (10%) Adjust pH if needed Water forInjection q.s. to 1 mL Solvent

We claim:
 1. A process for preparing a compound of the formula:

comprising the steps of: i.) Treatment of piperidine-4-carboxylic acidunder reductive amination conditions comprising formaldehyde and formicacid in water with subsequent treatment with aqueous HCl followed bywater distillation and acetonitrile addition, with repeateddilution/distillation until the water content is not more than 0.2% byKarl-Fischer analysis, to obtain solid 1-methylpiperidine-4-carboxylicacid hydrochloride; ii.) Treatment of 1-methylpiperidine-4-carboxylicacid hydrochloride with a chlorinating agent such as thionyl chloride inchlorobenzene to obtain 1-methylpiperidine-4-carboxylic acid chloride;iii.) Treatment of 1-methylpiperidine-4-carboxylic acid chloride withN,N-diethylamine in chlorobenzene containing triethylamine withsubsequent base wash and subsequent treatment with aqueous HCl inisopropanol to obtain solidN,N-diethyl-1-methyl-piperidine-4-carboxamide hydrate hydrochloride;iv.) Treatment of N,N-diethyl-1-methyl-piperidine-4-carboxamide hydratehydrochloride with a mineral base such as aqueous NaOH in a non-polarsolvent such as methyl-tert-butyl ether with subsequent water wash,phase separation, and distillation of the organic solvent until thewater content is not more than 0.1 weight t % by Karl Fischer analysisto obtain N,N-diethyl-1-methyl-piperidine-4-carboxamide; v.) Subsequenttreatment of N,N-diethyl-1-methyl-piperidine-4-carboxamide with(6-bromo-2-pyridyl)lithium in a non-polar organic solvent such asmethyl-tert-butyl ether with subsequent extraction of the resultingmixture with water and a suitable organic solvent such as n-butanol,phase separation, and repeated distillation of the organic solvent untilthe water content is not more than 0.2 weight % by Karl-Fischeranalysis, to obtain (6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone;vi.) Treatment of (6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanonewith aqueous HBr and subsequent extraction with n-butanol followed byrepeated distillation of the organic solvent until the water content isnot more than 0.3% by Karl-Fischer analysis, to obtain solid(6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone hydrobromide; vii.)Treatment of (6-bromo-2-pyridyl-1-methyl-4-piperidyl)methanonehydrobromide with a solution of NH₃ in ethylene glycol in the presenceof Cu₂O catalyst at about 80° C. for about 2 hr, with subsequent washeswith water, saturated aqueous NaCl, and 20% aqueous NaOH and subsequentextraction with a non-polar aprotic solvent such as methyl-tert-butylether, phase separation, and treatment of the organic phase with 5weight % carbon; viii.) Filtration of the above mixture, dilution with asuitable polar alcoholic solvent such as isopropanol, and repeateddistillation of the organic solvent until the water content is not morethan 0.2% by Karl-Fischer analysis, with subsequent treatment of theresulting residue with isopropanol, water, and 20 weight % HCl, whereinthe water concentration of the resulting slurry is at least 2%,filtration of the resulting slurry, and drying under vacuum at 40° C.for 16-24 hr to obtain solid(6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone dihydratedihydrochloride; ix.) Treatment of(6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone dihydratedihydrochloride in chlorobenzene with 6 weight/weight % NaOH in water atabout 54° C. for about 30 min, with subsequent phase separation andvacuum distillation of the aqueous solution to obtain(6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone; x.) Subsequenttreatment of (6-amino-2-pyridyl)-(1-methyl-4-piperidyl)methanone with2,4,6-trifluorobenzoic acid chloride in chlorobenzene at about 100° C.for about 4 hr, with subsequent cooling, charging with acetonitrile andheating the resulting slurry to 80° C. for about 1 hr, and subsequentcollection of the resulting solid by filtration, to obtain solid2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehydrochloride; xi.) Treatment of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehydrochloride with saturated aqueous Na₂CO₃ in methyl-tert-butyl ether;xii.) Treatment of the mixture of step xi above with SiO₂ withsubsequent filtration, treatment with carbon, filtration, andevaporation, dilution with ethanol, and distillation until the watercontent is not more than 1% by Karl-Fischer analysis, to obtain2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide;xiii.) Treatment of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidein ethanol with a solution of 0.5 equivalents succinic acid in ethanolat about 55° C. for not less than 3 hr at RT, and subsequent collectionof the solid by filtration, to obtain solid2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehemisuccinate.
 2. A process for preparing a compound of the formula

comprising the steps of: i.) Treatment of piperidine-4-carboxylic acidunder reductive amination conditions comprising formaldehyde and formicacid in water with subsequent treatment with aqueous HCl followed bywater distillation and acetonitrile addition, with repeateddilution/distillation until the water content is not more than 0.2% byKarl-Fischer analysis, to obtain solid 1-methylpiperidine-4-carboxylicacid hydrochloride; ii.) Treatment of 1-methylpiperidine-4-carboxylicacid hydrochloride with a chlorinating agent such as thionyl chloride inchlorobenzene obtain 1-methylpiperidine-4-carboxylic acid chloride;iii.) Treatment of 1-methylpiperidine-4-carboxylic acid chloride withN,N-diethylamine in chlorobenzene containing triethylamine withsubsequent base wash and subsequent treatment with aqueous HCl inisopropanol to obtain solidN,N-diethyl-1-methyl-piperidine-4-carboxamide hydrate hydrochloride;iv.) Treatment of N,N-diethyl-1-methyl-piperidine-4-carboxamide hydratehydrochloride with a mineral base such as aqueous NaOH in a non-polarsolvent such as methyl-tert-butyl ether with subsequent water wash,phase separation, and distillation of the organic solvent until thewater content is not more than 0.1 weight % by Karl Fischer analysis toobtain N,N-diethyl-1-methyl-piperidine-4-carboxamide; v.) Subsequenttreatment of N,N-diethyl-1-methyl-piperidine-4-carboxamide with(6-bromo-2-pyridyl)lithium in a non-polar organic solvent such asmethyl-tert-butyl ether with subsequent extraction of the resultingmixture with water and a suitable organic solvent such as n-butanol,phase separation, and repeated distillation of the organic solvent untilthe water content is not more than 0.2 weight % by Karl-Fischeranalysis, to obtain (6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone;vi.) Treatment of (6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanonewith aqueous HBr and subsequent extraction with n-butanol followed byrepeated distillation of the organic solvent until the water content isnot more than 0.3% by Karl-Fischer analysis, to obtain solid(6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone hydrobromide; vii.)Treatment of (6-bromo-2-pyridyl-1-methyl-4-piperidyl)methanonehydrobromide in a biphasic mixture of water and toluene with solid KOHfor about 3 hr with subsequent separation of the organic layer andevaporation of the solvent to obtain of(6-bromo-2-pyridyl-1-methyl-4-piperidyl)methanone; viii.) Treatment of(6-bromo-2-pyridyl-1-methyl-4-piperidyl)methanone with2,4,6-trifluorobenzamide in toluene containing K₂CO₃, water, Pd(OAc)₂,and Xantphos at about 70° C. for about 12 hr, until the(6-bromo-2-pyridyl)-(1-methyl-4-piperidyl)methanone content is not morethan 0.1% by HPLC, with subsequent dilution of the reaction mixture withwater and EtOAc, subsequent treatment with thiourea-modified silica gelat 60° C. for about 8 hr, with subsequent filtration to obtain asolution of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamide;ix.) Treatment of a solution of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidein EtOAc with a solution of about 0.5 equivalents of succinic aciddissolved in EtOH at 55° C. for about 3 hr, with subsequent cooling toRT over about 10 hr, and collection of the resulting solids byfiltration, to obtain solid2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehemisuccinate.
 3. A process of claim 1 or 2 wherein the reactions areperformed using batch processing methodology.
 4. A process of claim 3wherein the batch produced is at process scale.
 5. A process of claim 4wherein the batch produced is at least 1 kilogram.
 6. A process of claim4 wherein the batch produced is at least 10 kilograms.
 7. A process ofclaim 4 wherein the batch produced is at least 100 kilograms.
 8. Atablet produced by the process of claim 1 or 2 wherein the tabletcomprises 50 mg of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehemisuccinate.
 9. A tablet produced by the process of claim 1 or 2wherein the tablet comprises 100 mg of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehemisuccinate.
 10. A compound of the formula:


11. The compound of claim 10 which is crystalline.
 12. The compoundaccording to claim 11 characterized by an X-ray powder diffractionpattern using CuKα radiation having an intense peak at diffraction angle2-theta of 8.3° in combination with one or more of the peaks selectedfrom the group consisting of 16.6°, 23.5°, and 33.7° (±0.2°respectively).
 13. A compound of the formula:


14. The compound of claim 13 which is crystalline.
 15. The compoundaccording to claim 14 characterized by an X-ray powder diffractionpattern using CuKα radiation having an intense peak at diffraction angle2-theta of 26.2° in combination with one or more of the peaks selectedfrom the group consisting of 20.4°, 14.0°, and 17.9° (±0.2°respectively).
 16. A pharmaceutical composition comprising a compoundaccording to any one of claims 13 to 15 with one or morepharmaceutically acceptable carriers, diluents, or excipients.
 17. Thepharmaceutical composition of claim 16 further comprising acetic acid.18. A method of treating migraine in a patient comprising administeringto a patient in need of such treatment an effective amount of a compoundaccording to any one of claims 13 to
 15. 19. A tablet produced by theprocess of claim 1 or 2 wherein the tablet comprises 50 mg of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehemisuccinate and reactions are performed using batch processingmethodology and the batch produced is at least 1 kilogram.
 20. A tabletproduced by the process of claim 1 or 2 wherein the wherein the tabletcomprises 50 mg of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehemisuccinate and reactions are performed using batch processingmethodology and the batch produced is at least 10 kilograms.
 21. Atablet produced by the process of claim 1 or 2 wherein the wherein thetablet comprises 100 mg of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehemisuccinate and reactions are performed using batch processingmethodology and the batch produced is at least 1 kilogram.
 22. A tabletproduced by the process of claim 1 or 2 wherein the wherein the tabletcomprises 100 mg of2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-2-pyridyl]benzamidehemisuccinate and reactions are performed using batch processingmethodology and the batch produced is at least 10 kilograms.